| 名稱 | SRE-Luc/HEK293 |
| 型號 | CBP74030 |
| 報(bào)價(jià) | 50000 |
| 特點(diǎn) | SRE-Luc/HEK293，凍存條件：90% FBS+10% DMSO； |
產(chǎn)品搜索
相關(guān)文章
- 【產(chǎn)品推介】T細(xì)胞活化篩選模型
- 細(xì)胞株有關(guān)細(xì)胞培養(yǎng)污染的控制
- 細(xì)胞因子風(fēng)暴和自身免疫性炎癥治療新靶點(diǎn)GM-CSF
- 抗衰老全新靶點(diǎn)，抑制IL-11通路可延長哺乳動(dòng)物壽命
- Canine IL-31細(xì)胞篩選模型
- 5月是黑色素瘤意識月，盤點(diǎn)黑色素瘤研究進(jìn)展
- ATCC細(xì)胞的實(shí)驗(yàn)要點(diǎn)及說明
- HPV分子診斷標(biāo)準(zhǔn)品擴(kuò)容：精準(zhǔn)檢測，實(shí)力升級
- 基因守護(hù)者：抑癌基因TP53
- 心衰治療靶點(diǎn)：BNP/NPR1
聯(lián)系我們
聯(lián)系人：蔣經(jīng)理
電話：4008750250
號碼：
手機(jī)：18066071954
地址：南京市棲霞區(qū)緯地路9號
Email： zhangxiangwen@cobioer.com
電話：4008750250
號碼：
手機(jī)：18066071954
地址：南京市棲霞區(qū)緯地路9號
Email： zhangxiangwen@cobioer.com
產(chǎn)品展示 / PRODUCTS
藥靶細(xì)胞株 > 腫瘤免疫細(xì)胞株 > CBP74030SRE-Luc/HEK293
- 詳細(xì)內(nèi)容
| CBP74030 | |
| I. Background | |
| The MAPK/ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylate and activate ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The TCFs (Ternary Complex Factors), including Elk1, are among the best-characterized transcription factor substrates of ERK. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes. | |
| II. Description | |
| The SRE Reporter -HEK293 cell line is designed for monitoring the activity of the MAPK/ERK signaling pathway. The SRE Reporter -HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line. | |
| III. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | SRE-Luciferase |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | SRE cell line, MAPK reporter, ERK reporter |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+200ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
| |
Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose Response of Recombinant Human EGF in SRE/Luc HEK293 Cells (C5C16). | |
員_a.png)