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藥靶細(xì)胞株 > GPCR細(xì)胞株 > CBP71348Human ADORA3/CHO

Human ADORA3/CHO
名稱(chēng) Human ADORA3/CHO
型號(hào) CBP71348
報(bào)價(jià)
特點(diǎn) Human ADORA3/CHO 凍存條件:90% FBS+10% DMSO
  • 詳細(xì)內(nèi)容
CBP71348
I. Background

Extracellular adenosine mediates a multitude of biological effects, including wakefulness, antiarrythmia, bronchoconstriction and response to ischemia and oxidative stress.  A family of four G-protein coupled adrenoceptors, A1, A2A, A2B and A3, is responsible for these effects.  A3, which couples to Gi/o, is expressed in a wide range of human tissues, but most predominantly in the lung and liver.  Recent animal model studies have shown that A3 receptors play important roles in brain ischemia, immunosuppresion, and bronchospasm.  A3 receptor agonists and/or agonists may have important clinical value in the treatment of asthma and inflammation.  Mice lacking A3 receptors display reduced mast cell degranulation and bronchoconstriction in response to adenosine.


II. Introduction
Host Cell:

CHO

Stability:20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:90% FBS+10% DMSO
Culture Medium:F12K+10%FBS+5ug/ml puromycin
Mycoplasma Status:Negative
Storage:Liquid nitrogen immediately upon delivery
Application(s):

Functional assay for CCR2 receptor

Transducer:Gi

Ⅲ. Description of Host Cell Line
Organism:Hamster
Tissue:Ovary
Morphology: Epithelial
Growth Properties: Adherent

Ⅳ. Representative Data

Figure 1. NECA effect on ADORA3/CHO-K1 clone 5.





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